Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry
نویسندگان
چکیده
منابع مشابه
Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry
To monitor the kinetics of biological processes that take place within the minute time scale, simple and fast analytical methods are required. In this article, we present our discovery of an azide with an internal Cu(I)-chelating motif that enabled the development of the fastest protocol for Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) to date, and its application toward following the dyn...
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Dynamic alterations in cell surface glycosylation occur in numerous biological processes that involve cell-cell communication and cell migration. We report here imaging of cell surface glycosylation in live mice using double click chemistry. Cell surface glycans were metabolically labeled using peracetylated azido-labeled N-acetylgalactosamine and then reacted, in the first click reaction, with...
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Methods for profiling the activity of enzymes in vivo are needed to understand the role that these proteins and their endogenous regulators play in physiological and pathological processes. Recently, we introduced a tag-free strategy for activity-based protein profiling (ABPP) that utilizes the copper(I)-catalyzed azide-alkyne cycloaddition reaction ("click chemistry") to analyze the functional...
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ژورنال
عنوان ژورنال: Bioconjugate Chemistry
سال: 2014
ISSN: 1043-1802,1520-4812
DOI: 10.1021/bc400502d